A scientific exploration of auxin hormones and natural substances in propagating this rare succulent species
Nature makes some plants so rare and special that protecting and propagating them becomes a scientific adventure. Prometheum sempervivoides is a succulent plant with fleshy leaves and striking flowers that grows on the high-altitude rocky slopes of Turkey 3 . Ensuring the continuity of this species has become increasingly important for botanists due to the shrinking of its natural habitats and climate change.
Prometheum sempervivoides is endemic to specific regions of Turkey and is considered a rare species with limited distribution in its natural habitat.
In this article, we will explore the innovative research conducted by scientists to propagate this rare plant using leaf cuttings and the effects of auxin hormones and natural substances in this process.
Succulent plants like Prometheum sempervivoides have adapted to store water in their leaves, stems, or roots.
Vegetative propagation is the process of propagating a plant without using seeds, using vegetative parts (stem, leaf, or root) instead. New plants produced by this method are exact genetic copies of the parent plant 5 . This method is particularly valuable for rare plants like P. sempervivoides as it allows for the preservation of valuable genetic characteristics.
Allows cloning of plants with superior characteristics, maintaining genetic purity.
Shows faster development compared to seed propagation methods.
Guarantees the continuity of endangered species threatened by habitat loss.
In a study published in 2024, scientists conducted comprehensive research on the vegetative propagation of P. sempervivoides plants via leaf cuttings 1 . The main objective of the research was to determine the effects of different rooting applications and planting times on the plant's rooting performance.
Control (water only), commercial rooting hormone (three different doses: 0.25 g/l, 0.50 g/l and 1.0 g/l), cinnamon powder, and Aloe vera gel
Leaf cuttings planted immediately after collection (0 hours) and 24 hours later
Survival rate, number of rooted cuttings, callus formation, maximum root length, rooting rate, and rooting scale (1-5)
| Material | Function | Role in Research |
|---|---|---|
| Commercial Rooting Hormone | Contains auxin; stimulates cell division and root formation | Synthetic hormone source that promotes rooting |
| Cinnamon Powder | Natural antifungal and rooting aid | Natural rooting alternative |
| Aloe Vera Gel | Natural growth stimulant and moisture retainer | Natural rooting medium and growth regulator |
| Perlite | Rooting medium; provides drainage and aeration | Physical medium where cuttings were planted |
The results of the research revealed that rooting hormone applications, especially when applied at the highest dose (1.0 g/l), provided the most successful rooting 1 . This application was followed by cinnamon powder as a natural option. Aloe vera gel showed lower effectiveness.
| Application | Rooting Rate | Root Quality | Callus Formation |
|---|---|---|---|
| Control (Water) | Low | Weak | Limited |
| Rooting Hormone (1.0 g/l) | Highest | Excellent | Intensive |
| Cinnamon Powder | Medium | Good | Medium |
| Aloe Vera Gel | Low-Medium | Medium | Limited |
Comparative success rates of different rooting applications
| Score | Description | Observed Characteristics |
|---|---|---|
| 1 | Very Poor | No roots or very minimal |
| 2 | Poor | A few short roots |
| 3 | Medium | Several medium-length roots |
| 4 | Good | Well-developed root network |
| 5 | Excellent | Dense, well-developed root system |
Planting time was also found to have a significant effect on rooting. Leaf cuttings planted immediately after collection (0 hours) showed more successful rooting compared to those stored for 24 hours 1 . This finding demonstrates how critical the freshness of cuttings is for root formation.
This research has demonstrated that rare succulent species like Prometheum sempervivoides can be successfully propagated using leaf cuttings. While high-dose rooting hormone (1.0 g/l) was identified as the most effective rooting method, cinnamon powder as a natural alternative also showed promising results 1 .
The importance of this study stems not only from being a scientific achievement but also from its contribution to biodiversity conservation. Vegetative propagation methods are vital for passing on plant species threatened by climate change and habitat loss to future generations.
Protecting these fragile treasures of nature will be possible through the blending of scientific innovations and traditional knowledge. As in the case of P. sempervivoides, the successful propagation of each plant species is an important step taken toward preserving our natural heritage.